Design your Cas9 RNA guide and control the off-targets.
CRISPOR - CRISPr selectOR - is a program that helps design and evaluate target sites for use with the CRISPR/Cas9 system.
It uses the BWA algorithm to identify guide RNA sequences for CRISPR mediated genome editing. It searches for off-target sites (with and without mismatches), shows them in a table and annotates them with flanking genes.
CRISPOR currently includes more than 70 genomes ! To add your genome of interest, contact the CRISPOR web site manager:

For more information on principles of CRISPR-mediated genome editing, check the Addgene CRISPR guide:
8th annual meeting EFOR network, May 2nd and 3rd 2017
2nd - 3rd of May 2017
EFOR - Réseau d'´Étude Fonctionnelle chez les ORganismes modèles
Colloque OPAL : Place des méthodes de remplacement en expérimentation biologique
New plasmids for CRISPR/Cas9-mediated knock-in in zebrafish
The CRISPR Revolution
Place alternative methods in biological experimentation
Guidance on the environmental risk assessment of genetically modified animals
CRISPR Cas9: A novel approach to genetic engineering
Fish brain successfully transparized (clarity method) by the Tefor Core Facility
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