CRISPOR - CRISPr selectOR - is a program that helps design and evaluate target sites for use with the CRISPR/Cas9 system.
It uses the BWA algorithm to identify guide RNA sequences for CRISPR mediated genome editing. It searches for off-target sites (with and without mismatches), shows them in a table and annotates them with flanking genes.
CRISPOR currently includes more than 70 genomes ! To add your genome of interest, contact the CRISPOR web site manager: email@example.com.
For more information on principles of CRISPR-mediated genome editing, check the Addgene CRISPR guide: https://www.addgene.org/CRISPR/guide/